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Evaluation of Interferon-Gamma Polymorphisms as a Risk Factor in Feline Infectious Peritonitis Development in Non-Pedigree Cats-A Large Cohort Study.

  • Barker EN Langford Vets, University of Bristol, Langford BS40 5DU, UK.
  • Lait P Langford Vets, University of Bristol, Langford BS40 5DU, UK.
  • Ressel L Department of Veterinary Pathology and Public Health, Institute of Veterinary Science, University of Liverpool, Neston CH64 7TE, UK.
  • Blackwell EJ Bristol Veterinary School, University of Bristol, Langford BS40 5DU, UK.
  • Tasker S Bristol Veterinary School, University of Bristol, Langford BS40 5DU, UK.
  • Kedward-Dixon H Circa Healthcare, 116 Dundas Street, Edinburgh EH3 5DQ, UK.
  • Kipar A Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, CH-8057 Zurich, Switzerland.
  • Helps CR Langford Vets, University of Bristol, Langford BS40 5DU, UK.
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  • 2020-07-09
Published in:
  • Pathogens (Basel, Switzerland). - 2020
cohort study
feline coronavirus
gamma interferon
genetic risk factor
pyrosequencing
English Feline infectious peritonitis (FIP) is a common infectious cause of death in cats, with heritable host factors associated with altered risk of disease. To assess the role of feline interferon-gamma gene (fIFNG) variants in this risk, the allele frequencies of two single nucleotide polymorphisms (SNPs) (g.401 and g.408) were determined for non-pedigree cats either with confirmed FIP (n = 59) or from the general population (cats enrolled in a large lifetime longitudinal study; n = 264). DNA was extracted from buccal swabs or tissue samples. A pyrosequencing assay to characterize the fIFNG SNPs was designed, optimized and subsequently performed on all samples. Genotype and allele frequency were calculated for each population. Characterization of the target SNPs was possible for 56 of the cats with FIP and 263 of the cats from the general population. The SNPs were in complete linkage disequilibrium with each other. There was an association between FIP status and genotype (χ2; p = 0.028), with a reduced risk of developing FIP (χ2; p = 0.0077) associated with the genotype TT at both positions. These results indicate that, although fIFNG variants may be associated with altered risk of disease, the prevalence of individual variants within both populations limits application of their characterization to breeding purposes.
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  • English
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gold
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https://sonar.ch/global/documents/33489
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