Delivery of endocytosed proteins to the cell–division plane requires change of pathway from recycling to secretion

Richter, Sandra; Kientz, Marika; Brumm, Sabine; Nielsen, Mads Eggert; Park, Misoon; Gavidia, Richard; Krause, Cornelia; Voss, Ute; Beckmann, Hauke; Mayer, Ulrike; Stierhof, York-Dieter; Jürgens, Gerd

doi:10.7554/eLife.02131

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Journal article

Delivery of endocytosed proteins to the cell–division plane requires change of pathway from recycling to secretion

Richter, Sandra Department of Developmental Genetics, The Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany
Kientz, Marika Department of Developmental Genetics, The Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany
Brumm, Sabine Department of Developmental Genetics, The Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany
Nielsen, Mads Eggert Department of Developmental Genetics, The Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany
Park, Misoon Department of Developmental Genetics, The Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany
Gavidia, Richard Department of Developmental Genetics, The Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany
Krause, Cornelia Department of Developmental Genetics, The Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany
Voss, Ute Department of Developmental Genetics, The Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany
Beckmann, Hauke Department of Developmental Genetics, The Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany
Mayer, Ulrike Microscopy, The Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany
Stierhof, York-Dieter Microscopy, The Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany
Jürgens, Gerd Department of Developmental Genetics, The Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany

2014-4-8

Published in:

eLife. - eLife Sciences Publications, Ltd. - 2014, vol. 3

English Membrane trafficking is essential to fundamental processes in eukaryotic life, including cell growth and division. In plant cytokinesis, post-Golgi trafficking mediates a massive flow of vesicles that form the partitioning membrane but its regulation remains poorly understood. Here, we identify functionally redundant Arabidopsis ARF guanine-nucleotide exchange factors (ARF-GEFs) BIG1–BIG4 as regulators of post-Golgi trafficking, mediating late secretion from the trans-Golgi network but not recycling of endocytosed proteins to the plasma membrane, although the TGN also functions as an early endosome in plants. In contrast, BIG1-4 are absolutely required for trafficking of both endocytosed and newly synthesized proteins to the cell–division plane during cytokinesis, counteracting recycling to the plasma membrane. This change from recycling to secretory trafficking pathway mediated by ARF-GEFs confers specificity of cargo delivery to the division plane and might thus ensure that the partitioning membrane is completed on time in the absence of a cytokinesis-interphase checkpoint.

Language

English

Open access status

gold

Identifiers

DOI 10.7554/eLife.02131
ISSN 2050-084X

Persistent URL

https://sonar.ch/global/documents/34935

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