Journal article
Detergent-free membrane protein crystallization.
- Nollert P Department of Molecular Microbiology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056, Basel, Switzerland.
- Royant A
- Pebay-Peyroula E
- Landau EM
- 1999-09-03
Published in:
- FEBS letters. - 1999
Bacteriorhodopsins
Crystallization
Crystallography, X-Ray
Detergents
Halobacterium
Membrane Proteins
Purple Membrane
English
A comprehensive understanding of structure-function relationships of proteins requires their structures to be elucidated to high resolution. With most membrane proteins this has not been accomplished so far, mainly because of their notoriously poor crystallizability. Here we present a completely detergent-free procedure for the incorporation of a native purple membrane into a monoolein-based lipidic cubic phase, and subsequent crystallization of three-dimensional bacteriorhodopsin crystals therein. These crystals exhibit comparable X-ray diffraction quality and mosaicity, and identical crystal habit and space group to those of bacteriorhodopsin crystals that are grown from detergent-solubilized protein in cubic phase.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1016/s0014-5793(99)01014-5
- PMID 10471779
- Persistent URL
- https://sonar.ch/global/documents/36976
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