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Microfluidic Single-Cell Analysis with Affinity Beads.
Journal article

Microfluidic Single-Cell Analysis with Affinity Beads.

  • Werner M Institute of Chemical Sciences and Engineering, Swiss Federal Institute of Technology Lausanne (EPFL), 1015, Lausanne, Switzerland.
  • Palankar R Institute of Chemical Sciences and Engineering, Swiss Federal Institute of Technology Lausanne (EPFL), 1015, Lausanne, Switzerland.
  • Arm L Institute of Chemical Sciences and Engineering, Swiss Federal Institute of Technology Lausanne (EPFL), 1015, Lausanne, Switzerland.
  • Hovius R Institute of Chemical Sciences and Engineering, Swiss Federal Institute of Technology Lausanne (EPFL), 1015, Lausanne, Switzerland.
  • Vogel H Institute of Chemical Sciences and Engineering, Swiss Federal Institute of Technology Lausanne (EPFL), 1015, Lausanne, Switzerland.
  • 2015-02-03
Published in:
  • Small (Weinheim an der Bergstrasse, Germany). - 2015
affinity beads
microfluidics
optical tweezers
photochemical internalization
single-cell analysis
Cell Separation
Flow Cytometry
HEK293 Cells
Humans
Immunoassay
Microfluidics
Optical Tweezers
English A micrometer-sized affinity bead (red) is (i) taken up into a cell by phagocytosis, (ii) photochemically released from phagosomes, (iii) optically trapped by the cell, and (iv) isolated by cell lysis for subsequent analysis of captured intracellular analyte (green).
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/37841
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