Agrobacterium-mediated transformation of the white-rot fungus Physisporinus vitreus.
Schubert MEmpa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Applied Wood Materials, Lerchenfeldstrasse 5, CH-9014 St. Gallen, Switzerland. Electronic address: mark.schubert@empa.ch.
English
The biotechnologically important white-rot fungus Physisporinus vitreus was co-cultivated with Agrobacterium tumefaciens AGL-1 carrying plasmids with nourseothricin resistance as the selectable marker gene and red fluorescence protein as a visual marker. Mitotically stable transformed isolates were obtained showing red fluorescence protein activity.