Molecular Characterization of Divergent Closterovirus Isolates Infecting Ribes Species.
- Koloniuk I Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., Branišovská 31, 370 05 České Budějovice, Czech Republic. koloniuk@umbr.cas.cz.
- Thekke-Veetil T Department of Plant Pathology, Division of Agriculture, University of Arkansas System, Fayetteville, AR 72701, USA. thanujatv@gmail.com.
- Reynard JS Virology-Phytoplasmology Laboratory, Agroscope, 1260 Nyon, Switzerland. jean-sebastien.reynard@agroscope.admin.ch.
- Mavrič Pleško I Agricultural Institute of Slovenia, Hacquetova ulica 17, 1000 Ljubljana, Slovenia. irena.mavricplesko@kis.si.
- Přibylová J Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., Branišovská 31, 370 05 České Budějovice, Czech Republic. pribyl@umbr.cas.cz.
- Brodard J Virology-Phytoplasmology Laboratory, Agroscope, 1260 Nyon, Switzerland. justine.brodard@agroscope.admin.ch.
- Kellenberger I Virology-Phytoplasmology Laboratory, Agroscope, 1260 Nyon, Switzerland. isabelle.kellenberger@agroscope.admin.ch.
- Sarkisova T Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., Branišovská 31, 370 05 České Budějovice, Czech Republic. sarkisova@umbr.cas.cz.
- Špak J Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., Branišovská 31, 370 05 České Budějovice, Czech Republic. spak@umbr.cas.cz.
- Lamovšek J Agricultural Institute of Slovenia, Hacquetova ulica 17, 1000 Ljubljana, Slovenia. janja.lamovsek@kis.si.
- Massart S Plant Pathology Laboratory, TERRA-Gembloux Agro-Bio Tech, University of Liège, Passage des Déportés, 2, 5030 Gembloux, Belgium. sebastien.massart@uliege.be.
- Ho T Department of Plant Pathology, Division of Agriculture, University of Arkansas System, Fayetteville, AR 72701, USA. txho@uark.edu.
- Postman JD National Clonal Germplasm Repository, United States Department of Agriculture, Corvallis, OR 97333, USA. joseph.postman@ars.usda.gov.
- Tzanetakis IE Department of Plant Pathology, Division of Agriculture, University of Arkansas System, Fayetteville, AR 72701, USA. itzaneta@uark.edu.
- 2018-07-14
Published in:
- Viruses. - 2018
Ribes
closterovirus
currant
recombinants/recombination
Amino Acid Sequence
Closterovirus
Genetic Variation
Genome, Viral
Genomics
High-Throughput Nucleotide Sequencing
Open Reading Frames
Phylogeny
Plant Diseases
RNA, Viral
Recombination, Genetic
Ribes
English
Five isolates of a new member of the family Closteroviridae, tentatively named blackcurrant leafroll-associated virus 1 (BcLRaV-1), were identified in the currant. The 17-kb-long genome codes for 10 putative proteins. The replication-associated polyprotein has several functional domains, including papain-like proteases, methyltransferase, Zemlya, helicase, and RNA-dependent RNA polymerase. Additional open reading frames code for a small protein predicted to integrate into the host cell wall, a heat-shock protein 70 homolog, a heat-shock protein 90 homolog, two coat proteins, and three proteins of unknown functions. Phylogenetic analysis showed that BcLRaV-1 is related to members of the genus Closterovirus, whereas recombination analysis provided evidence of intraspecies recombination.
- Language
-
- English
- Open access status
- gold
- Identifiers
-
- DOI 10.3390/v10070369
- PMID 30002359
- Persistent URL
- https://sonar.ch/global/documents/39634
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