Measuring SARS-CoV-2 neutralizing antibody activity using pseudotyped and chimeric viruses
- Schmidt, Fabian ORCID Laboratory of Retrovirology, The Rockefeller University, New York, NY
- Weisblum, Yiska ORCID Laboratory of Retrovirology, The Rockefeller University, New York, NY
- Muecksch, Frauke ORCID Laboratory of Retrovirology, The Rockefeller University, New York, NY
- Hoffmann, Hans-Heinrich ORCID Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY
- Michailidis, Eleftherios ORCID Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY
- Lorenzi, Julio C.C. ORCID Laboratory of Molecular Immunology, The Rockefeller University, New York, NY
- Mendoza, Pilar ORCID Laboratory of Molecular Immunology, The Rockefeller University, New York, NY
- Rutkowska, Magdalena ORCID Laboratory of Retrovirology, The Rockefeller University, New York, NY
- Bednarski, Eva ORCID Laboratory of Retrovirology, The Rockefeller University, New York, NY
- Gaebler, Christian ORCID Laboratory of Molecular Immunology, The Rockefeller University, New York, NY
- Agudelo, Marianna ORCID Laboratory of Molecular Immunology, The Rockefeller University, New York, NY
- Cho, Alice ORCID Laboratory of Molecular Immunology, The Rockefeller University, New York, NY
- Wang, Zijun ORCID Laboratory of Molecular Immunology, The Rockefeller University, New York, NY
- Gazumyan, Anna ORCID Laboratory of Molecular Immunology, The Rockefeller University, New York, NY
- Cipolla, Melissa ORCID Laboratory of Molecular Immunology, The Rockefeller University, New York, NY
- Caskey, Marina ORCID Laboratory of Molecular Immunology, The Rockefeller University, New York, NY
- Robbiani, Davide F. ORCID Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland
- Nussenzweig, Michel C. ORCID Howard Hughes Medical Institute, The Rockefeller University, New York, NY
- Rice, Charles M. ORCID Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY
- Hatziioannou, Theodora ORCID Laboratory of Retrovirology, The Rockefeller University, New York, NY
- Bieniasz, Paul D. ORCID Howard Hughes Medical Institute, The Rockefeller University, New York, NY
- 2020-7-21
Published in:
- Journal of Experimental Medicine. - Rockefeller University Press. - 2020, vol. 217, no. 11
English
The emergence of SARS-CoV-2 and the ensuing explosive epidemic of COVID-19 disease has generated a need for assays to rapidly and conveniently measure the antiviral activity of SARS-CoV-2–specific antibodies. Here, we describe a collection of approaches based on SARS-CoV-2 spike-pseudotyped, single-cycle, replication-defective human immunodeficiency virus type-1 (HIV-1), and vesicular stomatitis virus (VSV), as well as a replication-competent VSV/SARS-CoV-2 chimeric virus. While each surrogate virus exhibited subtle differences in the sensitivity with which neutralizing activity was detected, the neutralizing activity of both convalescent plasma and human monoclonal antibodies measured using each virus correlated quantitatively with neutralizing activity measured using an authentic SARS-CoV-2 neutralization assay. The assays described herein are adaptable to high throughput and are useful tools in the evaluation of serologic immunity conferred by vaccination or prior SARS-CoV-2 infection, as well as the potency of convalescent plasma or human monoclonal antibodies.
- Language
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- English
- Open access status
- hybrid
- Identifiers
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- DOI 10.1084/jem.20201181
- ISSN 0022-1007
- Persistent URL
- https://sonar.ch/global/documents/41099
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