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Effects of the cell geometry and operating parameters on the performance of an external contactless conductivity detector for microchip electrophoresis.
Journal article

Effects of the cell geometry and operating parameters on the performance of an external contactless conductivity detector for microchip electrophoresis.

  • Kubán P Department of Chemistry, University of Basel, Spitalstrasse 51, 4004 Basel, Switzerland.
  • Hauser PC
  • 2005-03-26
Published in:
  • Lab on a chip. - 2005
Cations
Electric Conductivity
Electrodes
Electrolytes
Electrophoresis, Capillary
Electrophoresis, Microchip
Equipment Design
Sensitivity and Specificity
Surface Properties
Time Factors
English Quantitative data on the effect of the electrode geometry on the signal strength and the signal-to-noise ratio is given. The measurements are affected by the unavoidable presence of stray capacitance. Best results are achieved for short and narrow electrodes arranged in an antiparallel configuration and separated by a minimal gap, which determines the dimensions of the actual detection volume. Limits of detection between 150 and 250 microg l(-1) and separation efficiencies from 13,000 to 17,000 theoretical plates were achieved for six inorganic cations (NH(4)(+), K(+), Ca(2+), Na(+), Mg(2+)and Li(+)) with electrodes of 1 mm width and a detection gap of 0.5 mm (separation channel length: 7.5 cm) when operating the detector at 20 V(pp) and 500 kHz. The analyses of all major inorganic cations in tap and rain water samples were demonstrated for the first time in microchip electrophoresis with contactless conductivity detection.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/45559
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