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Opposing chromatin remodelers control transcription initiation frequency and start site selection.
Journal article

Opposing chromatin remodelers control transcription initiation frequency and start site selection.

  • Kubik S Department of Molecular Biology and Institute of Genetics and Genomics of Geneva (iGE3), Geneva, Switzerland.
  • Bruzzone MJ Department of Molecular Biology and Institute of Genetics and Genomics of Geneva (iGE3), Geneva, Switzerland.
  • Challal D Institut Jacques Monod, CNRS-Université Paris Diderot, Paris, France.
  • Dreos R Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
  • Mattarocci S Department of Molecular Biology and Institute of Genetics and Genomics of Geneva (iGE3), Geneva, Switzerland.
  • Bucher P Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
  • Libri D Institut Jacques Monod, CNRS-Université Paris Diderot, Paris, France.
  • Shore D Department of Molecular Biology and Institute of Genetics and Genomics of Geneva (iGE3), Geneva, Switzerland. David.Shore@unige.ch.
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  • 2019-08-07
Published in:
  • Nature structural & molecular biology. - 2019
Chromatin Assembly and Disassembly
DNA, Fungal
DNA, Intergenic
Macromolecular Substances
Micrococcal Nuclease
Nucleosomes
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Transcription Initiation Site
Transcription Initiation, Genetic
English Precise nucleosome organization at eukaryotic promoters is thought to be generated by multiple chromatin remodeler (CR) enzymes and to affect transcription initiation. Using an integrated analysis of chromatin remodeler binding and nucleosome occupancy following rapid remodeler depletion, we investigated the interplay between these enzymes and their impact on transcription in yeast. We show that many promoters are affected by multiple CRs that operate in concert or in opposition to position the key transcription start site (TSS)-associated +1 nucleosome. We also show that nucleosome movement after CR inactivation usually results from the activity of another CR and that in the absence of any remodeling activity, +1 nucleosomes largely maintain their positions. Finally, we present functional assays suggesting that +1 nucleosome positioning often reflects a trade-off between maximizing RNA polymerase recruitment and minimizing transcription initiation at incorrect sites. Our results provide a detailed picture of fundamental mechanisms linking promoter nucleosome architecture to transcription initiation.
Language
  • English
Open access status
green
Identifiers
Persistent URL
https://sonar.ch/global/documents/46459
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