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Curvature-dependent constraints drive remodeling of epithelia.

  • Maechler FA Department of Biochemistry, University of Geneva, CH-1211 Geneva, Switzerland.
  • Allier C CEA, LETI, DTBS, LISA, Université Grenoble Alpes, F-38000 Grenoble, France.
  • Roux A Department of Biochemistry, University of Geneva, CH-1211 Geneva, Switzerland aurelien.roux@unige.ch caterina.tomba@unige.ch.
  • Tomba C Department of Biochemistry, University of Geneva, CH-1211 Geneva, Switzerland aurelien.roux@unige.ch caterina.tomba@unige.ch.
  • 2018-12-23
Published in:
  • Journal of cell science. - 2019
3D cell culture
Bioengineering
Cylindrical confinement
Epithelial morphogenesis
Alginates
Animals
Biomechanical Phenomena
Cell Adhesion
Cell Culture Techniques
Cell Line
Cell Movement
Cells, Immobilized
Collagen
Dogs
Drug Combinations
Epithelial Cells
Laminin
Madin Darby Canine Kidney Cells
Mechanotransduction, Cellular
Mice
Organ Specificity
Proteoglycans
English Epithelial tissues function as barriers that separate the organism from the environment. They usually have highly curved shapes, such as tubules or cysts. However, the processes by which the geometry of the environment and the cell's mechanical properties set the epithelium shape are not yet known. In this study, we encapsulated two epithelial cell lines, MDCK and J3B1A, into hollow alginate tubes and grew them under cylindrical confinement forming a complete monolayer. MDCK monolayers detached from the alginate shell at a constant rate, whereas J3B1A monolayers detached at a low rate unless the tube radius was reduced. We showed that this detachment is driven by contractile stresses in the epithelium and can be enhanced by local curvature. This allows us to conclude that J3B1A cells exhibit smaller contractility than MDCK cells. Monolayers inside curved tubes detach at a higher rate on the outside of a curve, confirming that detachment is driven by contraction.
Language
  • English
Open access status
hybrid
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https://sonar.ch/global/documents/46506
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