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Isolation and Antimicrobial Activity of Coumarin Derivatives from Fruits of Peucedanum luxurians Tamamsch.

  • Widelski J Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, Chodzki, 120-093 Lublin, Poland. jwidelski@pharmacognosy.org.
  • Luca SV Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, Chodzki, 120-093 Lublin, Poland. simon-vlad.v.luca@d.umfiasi.ro.
  • Skiba A Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, Chodzki, 120-093 Lublin, Poland. skiba.adrianna@gmail.com.
  • Chinou I Department of Pharmacognosy and Chemistry of Natural Products, School of Pharmacy, University of Athens, Zografou, 15771 Athens, Greece. ichinou@pharm.uoa.gr.
  • Marcourt L School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, CMU, 1, Rue Michel Servet, 1211 Geneva 4, Switzerland. Laurence.Marcourt@unige.ch.
  • Wolfender JL School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, CMU, 1, Rue Michel Servet, 1211 Geneva 4, Switzerland. Jean-Luc.Wolfender@unige.ch.
  • Skalicka-Wozniak K Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, Chodzki, 120-093 Lublin, Poland. kskalicka@pharmacognosy.org.
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  • 2018-05-23
Published in:
  • Molecules (Basel, Switzerland). - 2018
Apiaceae
Peucedanum luxurians Tamamsch.
accelerated solvent extraction
countercurrent chromatography
natural products
purification
Anti-Infective Agents
Apiaceae
Coumarins
Countercurrent Distribution
Fruit
Gram-Negative Bacteria
Gram-Positive Bacteria
Microbial Sensitivity Tests
Molecular Structure
Plant Components, Aerial
Plant Extracts
English As a continuation of searching for phytoconstituents that act as promising agents for antimicrobial therapy, rare coumarins were isolated from fruits of Peucedanum luxurians and tested. In a first step, the content of major compounds in the aerial parts and fruits of P. luxurians were compared. The results clearly showed that the fruits with dichloromethane as a solvent yielded, in most cases, higher concentrations of almost all the analyzed coumarins than the aerial parts, with peucedanin detected as the most abundant compound with a concentration of 4563.94 ± 3.35 mg/100 g. Under this perspective, the dichloromethane extract from the fruits of P. luxurians was further submitted to high performance countercurrent chromatography with a mixture of n-hexane, ethyl acetate, methanol, and water 6:5:6:5 (v/v). Combination of HPCCC and prep-HPLC yielded 6',7'-dihydroxybergamottin (1), officinalin (2), stenocarpin isobutyrate (3), officinalin isobutyrate (4), 8-methoxypeucedanin (5), and peucedanin (6). Isolated compounds were tested against several Gram-positive and Gram-negative bacteria strains. 6',7'-Dihydroxybergamottin, peucedanin, and officinalin isobutyrate appeared to be the most active against all tested bacteria strains with minimum inhibitory concentration (MIC) values between 1.20 and 4.80 mg/mL. To the best of our knowledge, this is the first report about countercurrent isolation of mentioned coumarins, as well as the first information about their antimicrobial activity.
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  • English
Open access status
gold
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Persistent URL
https://sonar.ch/global/documents/46597
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