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Polyelectrolyte nanocomplexes based on chitosan derivatives for wound healing application.

  • Patrulea V School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 1211 Geneva, Switzerland.
  • Laurent-Applegate LA University Hospital of Lausanne (CHUV-UNIL), Department of Musculoskeletal Medicine, EPCR/02/ch Croisettes 22, 1066 Epalinges, Switzerland.
  • Ostafe V West University of Timisoara, Department of Chemistry, Timisoara 300115, Romania; West University of Timisoara, Advanced Environmental Research Laboratories, Timisoara 300086, Romania.
  • Borchard G School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 1211 Geneva, Switzerland.
  • Jordan O School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 1211 Geneva, Switzerland. Electronic address: Olivier.Jordan@unige.ch.
  • 2019-05-16
Published in:
  • European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V. - 2019
Chitosan derivative
Hydrogel
Polyelectrolyte
RGDC
Wound healing
Cell Adhesion
Cell Line
Chitosan
Fibroblasts
Humans
Hydrogels
Hydrogen-Ion Concentration
Oligopeptides
Polyelectrolytes
Polymers
Skin
Wound Healing
English Wound healing, when compromised, may be guided by biological cues such as Arg-Gly-Asp (RGD), a peptide known to induce cell adhesion and migration, eventually combined with adapted nanocarriers. Three different formulations were prepared and investigated in vitro for topical application. All formulations were based on carboxylated and trimethylated chitosan (CMTMC) displaying RGD. The polyelectrolyte nanocomplexes were prepared by mixing two oppositely charged polymers of CMTMC and chondroitin sulfate at different polymer ratios and subsequently characterized by dynamic light scattering and scanning electron microscopy. Hydrogels and foams with a high concentration of RGD-functionalized chitosan (3%) and hyaluronic acid (1.5%) that formed gel-embedded nanocomplexes were developed. In vitro assays showed absence of toxicity, ability to promote proliferation over 7 days and promotion of migration of human dermal fibroblasts treated with any of our formulations. These formulations were shown to be suitable for easy topical application and have the potential to accelerate wound healing.
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  • English
Open access status
green
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https://sonar.ch/global/documents/46755
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