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Towards early-warning gene signature of Chlamydomonas reinhardtii exposed to Hg-containing complex media.

  • Beauvais-Flück R Department F.-A. Forel for environmental and aquatic sciences, Earth and Environmental Sciences, Faculty of Sciences, University of Geneva, 66, boulevard Carl-Vogt, CH-1211, Geneva 4, Switzerland.
  • Slaveykova VI Department F.-A. Forel for environmental and aquatic sciences, Earth and Environmental Sciences, Faculty of Sciences, University of Geneva, 66, boulevard Carl-Vogt, CH-1211, Geneva 4, Switzerland.
  • Ulf S Department of Forest Ecology and Management, Swedish University of Agricultural Sciences, 901 83, Umeå, Sweden.
  • Cosio C Department F.-A. Forel for environmental and aquatic sciences, Earth and Environmental Sciences, Faculty of Sciences, University of Geneva, 66, boulevard Carl-Vogt, CH-1211, Geneva 4, Switzerland. Electronic address: claudia.cosio@unige.ch.
  • 2019-07-29
Published in:
  • Aquatic toxicology (Amsterdam, Netherlands). - 2019
Copper
Dissolved organic matter
Microalgae
Transcriptomics
Uptake
Animals
Biological Availability
Cadmium
Chlamydomonas reinhardtii
Environmental Exposure
Female
Gene Expression Profiling
Humic Substances
Mercury
Methylmercury Compounds
Mitochondria
NAD
Oncorhynchus mykiss
Oxygen Consumption
Reactive Oxygen Species
Temperature
Water Pollutants, Chemical
English The potential of using gene expression signature as a biomarker of toxicants exposure was explored in the microalga Chlamydomonas reinhardtii exposed 2 h to mercury (Hg) as inorganic mercury (IHg) and methyl mercury (MeHg) in presence of copper (Cu) and Suwannee River Humic Acid (SRHA). Total cellular Hg (THg = IHg + MeHg) decreased in presence of SRHA for 0.7 nM IHg and 0.4 nM MeHg, but increased for 70 nM IHg exposure. In mixtures of IHg + MeHg and (IHg or MeHg) + Cu, SRHA decreased THg uptake, except for 0.7 nM IHg + 0.4 nM MeHg which was unchanged (p-value>0.05). In the absence of SRHA, 0.5 μM Cu strongly decreased intracellular THg concentration for 70 nM IHg, while it had no effect for 0.7 nM IHg and 0.4 nM MeHg. The expression of single transcripts was not correlated with measured THg uptake, but a subset of 60 transcripts showed signatures specific to the exposed metal(s) and was congruent with exposure concentration. Notably, the range of fold change values of this subset correlated with THg bioaccumulation with a two-slope pattern in line with [THg]intra/[THg]med ratios. Gene expression signature seems a promising approach to complement chemical analyses to assess bioavailability of toxicants in presence of other metals and organic matter.
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  • English
Open access status
green
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https://sonar.ch/global/documents/46768
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