Journal article
Activation of the macroautophagy pathway by Yersinia enterocolitica promotes intracellular multiplication and egress of yersiniae from epithelial cells.
- Valencia Lopez MJ Institute for Medical Microbiology, Virology and Hygiene, University, Medical Center Eppendorf, Hamburg, Germany.
- Schimmeck H Institute for Medical Microbiology, Virology and Hygiene, University, Medical Center Eppendorf, Hamburg, Germany.
- Gropengießer J Institute for Medical Microbiology, Virology and Hygiene, University, Medical Center Eppendorf, Hamburg, Germany.
- Middendorf L Institute for Medical Microbiology, Virology and Hygiene, University, Medical Center Eppendorf, Hamburg, Germany.
- Quitmann M Institute for Medical Microbiology, Virology and Hygiene, University, Medical Center Eppendorf, Hamburg, Germany.
- Schneider C Heinrich-Pette-Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.
- Holstermann B Heinrich-Pette-Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.
- Wacker R Institute for Cell Biology, University of Bern, Bern, Switzerland.
- Heussler V Institute for Cell Biology, University of Bern, Bern, Switzerland.
- Reimer R Heinrich-Pette-Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.
- Aepfelbacher M Institute for Medical Microbiology, Virology and Hygiene, University, Medical Center Eppendorf, Hamburg, Germany.
- Ruckdeschel K Institute for Medical Microbiology, Virology and Hygiene, University, Medical Center Eppendorf, Hamburg, Germany.
- 2019-05-18
Published in:
- Cellular microbiology. - 2019
Yersinia
autophagy
infection
microbial-cell interaction
Animals
Autophagosomes
Cell Death
Epithelial Cells
HeLa Cells
Host Microbial Interactions
Humans
Lysosomes
Mice
Microscopy, Electron, Transmission
Microtubule-Associated Proteins
Vacuoles
Yersinia enterocolitica
English
The virulence strategy of pathogenic Yersinia spp. involves cell-invasive as well as phagocytosis-preventing tactics to enable efficient colonisation of the host organism. Enteropathogenic yersiniae display an invasive phenotype in early infection stages, which facilitates penetration of the intestinal mucosa. Here we show that invasion of epithelial cells by Yersinia enterocolitica is followed by intracellular survival and multiplication of a subset of ingested bacteria. The replicating bacteria were enclosed in vacuoles with autophagy-related characteristics, showing phagophore formation, xenophagy, and recruitment of cytoplasmic autophagosomes to the bacteria-containing compartments. The subsequent fusion of these vacuoles with lysosomes and concomitant vesicle acidification were actively blocked by Yersinia. This resulted in increased intracellular proliferation and detectable egress of yersiniae from infected cells. Notably, deficiency of the core autophagy machinery component FIP200 impaired the development of autophagic features at Yersinia-containing vacuoles as well as intracellular replication and release of bacteria to the extracellular environment. These results suggest that Y. enterocolitica may take advantage of the macroautophagy pathway in epithelial cells to create an autophagosomal niche that supports intracellular bacterial survival, replication, and, eventually, spread of the bacteria from infected cells.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1111/cmi.13046
- PMID 31099152
- Persistent URL
- https://sonar.ch/global/documents/46828
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