Metabolomics for early detection of stress in freshwater alga Poterioochromonas malhamensis exposed to silver nanoparticles.
- Liu W Department F.-A. Forel for Environmental and Aquatic Sciences, Environmental Biogeochemistry and Ecotoxicology, Faculty of Sciences, Earth and Environment Sciences, University of Geneva, Uni Carl Vogt,66 Blvd Carl-Vogt, 1211, Geneva, Switzerland.
- Majumdar S Bren School of Environmental Science and Management, University of California, Santa Barbara, CA, 93106-5131, USA.
- Li W Bren School of Environmental Science and Management, University of California, Santa Barbara, CA, 93106-5131, USA.
- Keller AA Bren School of Environmental Science and Management, University of California, Santa Barbara, CA, 93106-5131, USA.
- Slaveykova VI Department F.-A. Forel for Environmental and Aquatic Sciences, Environmental Biogeochemistry and Ecotoxicology, Faculty of Sciences, Earth and Environment Sciences, University of Geneva, Uni Carl Vogt,66 Blvd Carl-Vogt, 1211, Geneva, Switzerland. vera.slaveykova@unige.ch.
- 2020-11-26
Published in:
- Scientific reports. - 2020
English
Silver nanoparticles (AgNPs) are one of the most used engineered nanomaterials. Despite progress in assessing their environmental implications, knowledge gaps exist concerning the metabolic perturbations induced by AgNPs on phytoplankton, essential organisms in global biogeochemical cycles and food-web dynamics. We combine targeted metabolomics, biouptake and physiological response studies to elucidate metabolic perturbations in alga Poterioochromonas malhamensis induced by AgNPs and dissolved Ag. We show time-dependent perturbation of the metabolism of amino acids, nucleotides, fatty acids, tricarboxylic acids, photosynthesis and photorespiration by both Ag-treatments. The results suggest that dissolved Ag ions released by AgNPs are the major toxicity driver; however, AgNPs internalized in food vacuoles contributed to the perturbation of amino acid metabolism, TCA cycle and oxidative stress. The metabolic perturbations corroborate the observed physiological responses. We highlight the potential of metabolomics as a tool for understanding the molecular basis for these metabolic and physiological changes, and for early detection of stress.
- Language
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- English
- Open access status
- gold
- Identifiers
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- DOI 10.1038/s41598-020-77521-0
- PMID 33239722
- Persistent URL
- https://sonar.ch/global/documents/46900
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