Quantitative determination of isoflurane enantiomers in blood samples during and after surgery via headspace gas chromatography-mass spectrometry.

Juza M; Jakubetz H; Hettesheimer H; Schurig V

doi:10.1016/s0378-4347(99)00409-0

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Journal article

Quantitative determination of isoflurane enantiomers in blood samples during and after surgery via headspace gas chromatography-mass spectrometry.

Juza M Laboratorium für Technische Chemie, ETH Zürich, Switzerland. juza@tech.chem.ethz.ch
Jakubetz H
Hettesheimer H
Schurig V

2000-01-12

Published in:

Journal of chromatography. B, Biomedical sciences and applications. - 1999

Gas Chromatography-Mass Spectrometry

Ophthalmologic Surgical Procedures

English The quantitative analysis of the chiral volatile anesthetic isoflurane (1) for biomedical applications by means of enantioselective gas chromatography (mass sensitive detector, selected ion monitoring) was studied. Two methods for the quantification of the enantiomers in blood samples drawn during and after narcosis were compared. Either the isomeric enflurane (2) was selected as an internal standard or a single enantiomer of 1 was used for the standard addition method, an approach referred to as 'enantiomer labeling'. Concentrations up to 0.3 micromol/l of the single enantiomers could be differentiated two days after anesthesia. The presented data imply that the body clearance for (+)-(S)-1 and (-)-(R)-1 proceeds to a measurable degree of enantioselectivity.

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