Back
Bacterial response to acetate challenge: a comparison of tolerance among species.
Journal article

Bacterial response to acetate challenge: a comparison of tolerance among species.

  • 2000-09-01
Published in:
  • Applied microbiology and biotechnology. - 2000
Acetates
Acetobacter
Bacteriological Techniques
Culture Media
Escherichia coli
Fermentation
Gluconobacter
Glucose
Glycerol
Lactobacillus
Species Specificity
Staphylococcus
English Although acetate formation and tolerance are important criteria for various aspects of biotechnological process development, available studies on acetate tolerance in different species are disparate. We evaluate the response of eight bacterial strains, including two variants of Escherichia coli, two variants of Staphylococcus capitis, and one each of Acetobacter aceti, Gluconobacter suboxydans, Lactobacillus acetotolerans, and L. bulgaricus, to acetate challenges under identical conditions. Our findings were: (1) wild-type organisms of species that are considered tolerant of acetate perform only slightly better than E. coli in unadapted shaker cultures; (2) the ability to tolerate acetate is strongly dependent on the carbon source, and is, especially for E. coli, much greater on glycerol than on glucose; (3) respiration is not as important to acetate tolerance in E. coli and S. capitis as has been reported for the acetic acid bacteria; (4) S. capitis was the least affected by acetate under all conditions and grew at up to 44 g/l acetate without any preconditioning; and (5) qualitative high-throughput screening of growth characteristics can be achieved with relatively inexpensive multiwell plate readers.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/46990
Statistics
Document views: 38 File downloads: