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Membrane topology of peripheral myelin protein 22.
Journal article

Membrane topology of peripheral myelin protein 22.

  • Taylor V Institute of Cell Biology, Department of Biology, Swiss Federal Institute of Technology, ETH-Hönggerberg, Zürich, Switzerland.
  • Zgraggen C
  • Naef R
  • Suter U
  • 2000-09-26
Published in:
  • Journal of neuroscience research. - 2000
Alkaline Phosphatase
Animals
COS Cells
Cell Membrane
Cells, Cultured
Computer Simulation
Endopeptidase K
Flow Cytometry
GPI-Linked Proteins
Hemagglutinins
Humans
Immunohistochemistry
Isoenzymes
Microsomes
Models, Molecular
Myelin Proteins
Protein Structure, Tertiary
Protein Transport
Rats
Receptors, Interleukin-2
Recombinant Fusion Proteins
Schwann Cells
Transfection
English Peripheral myelin protein 22 (PMP22) is a structural component of compact peripheral nerve myelin and is likely to play a role in the modulation of cell proliferation and cell spreading. Molecular genetics revealed that mutations affecting the PMP22 gene are responsible for the most common forms of hereditary motor and sensory neuropathies in humans. Computer analysis predicts a tetraspan-membrane structure for the PMP22 protein. We have assessed the topology of PMP22 experimentally using chimeric proteins consisting of different PMP22 domains fused to reporter genes and internally tagged molecules. Based on in vitro transcription/translation assays and immunohistochemical analysis of transfected cells, we propose that PMP22 can adopt a non-tetraspan topology that has functional implications in normal and disease processes.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/46991
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