Journal article
Trigonal crystals of porcine mitochondrial aspartate aminotransferase.
- Izard T Department of Structural Biology, University of Basel, Switzerland.
- Fol B
- Pauptit RA
- Jansonius JN
- 1990-10-05
Published in:
- Journal of molecular biology. - 1990
English
Crystals suitable for X-ray analysis of porcine mitochondrial aspartate aminotransferase in the closed conformation were obtained after the apoenzyme was reconstituted with N-5'-phosphopyridoxyl-L-aspartate, an inhibitor in which the cofactor is covalently bound to the substrate. This results in a crystal form that has not been encountered previously in studies of aspartate aminotransferases. The crystals belong to the trigonal space group P3121 (or the enantiomeric P3221) with unit cell dimensions alpha = b = 202.0 A, c = 58.0 A, alpha = beta = 90 degrees, gamma = 120 degrees and contain one dimer in the asymmetric unit.
- Language
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- English
- Open access status
- closed
- Identifiers
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- DOI 10.1016/s0022-2836(05)80355-9
- PMID 2231709
- Persistent URL
- https://sonar.ch/global/documents/47036
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