Bradyrhizobium japonicum NnrR, a denitrification regulator, expands the FixLJ-FixK2 regulatory cascade.
- Mesa S Institute of Microbiology, Eidgenössische Technische Hochschule, CH-8092 Zürich, Switzerland. mesam@micro.biol.ethz.ch
- Bedmar EJ
- Chanfon A
- Hennecke H
- Fischer HM
- 2003-06-19
Published in:
- Journal of bacteriology. - 2003
Bacterial Proteins
Bradyrhizobium
Gene Expression Regulation, Bacterial
Hemeproteins
Histidine Kinase
Iron-Sulfur Proteins
Molecular Sequence Data
Mutation
Nitric Oxide
Nitrites
Oxidation-Reduction
Transcription, Genetic
English
In Bradyrhizobium japonicum, a gene named nnrR was identified which encodes a protein with high similarity to FNR/CRP-type transcriptional regulators. Mutant strains carrying an nnrR null mutation were unable to grow anaerobically in the presence of nitrate or nitrite, and they lacked both nitrate and nitrite reductase activities. Anaerobic activation of an nnrR'-'lacZ fusion required FixLJ and FixK(2). In turn, N oxide-mediated induction of nir and nor genes encoding nitrite and nitric oxide reductase, respectively, depended on NnrR. Thus, NnrR expands the FixLJ-FixK(2) regulatory cascade by an additional control level which integrates the N oxide signal required for maximal induction of the denitrification genes.
- Language
-
- English
- Open access status
- bronze
- Identifiers
-
- DOI 10.1128/jb.185.13.3978-3982.2003
- PMID 12813094
- Persistent URL
- https://sonar.ch/global/documents/47094
Statistics
Document views: 21
File downloads:
- Full-text: 0