Journal article
Functional expression of arginine kinase improves recovery from pH stress of Escherichia coli.
- Canonaco F Institute of Biotechnology, ETH Zürich, CH-8093 Zürich, Switzerland.
- Schlattner U
- Wallimann T
- Sauer U
- 2003-08-02
Published in:
- Biotechnology letters. - 2003
Animals
Arginine Kinase
Cloning, Molecular
Escherichia coli
Homeostasis
Horseshoe Crabs
Hydrogen-Ion Concentration
Recombinant Proteins
Stress, Physiological
English
Acid stress in Escherichia coli involves a complex resource- and energy-consuming response mechanism. By overexpression of arginine kinase from Limulus polyphemus in E. coli, we improved the recovery from a transient pH stress. While wild type E. coli resumed growth after a transient pH reduction to pH 3 for 1 h with a rate that was 25% lower than before the stress, the arginine kinase expressing strain continued to grow as rapidly as before. This effect is presumably caused by the physiological function of arginine kinase as a short term energy buffer in the form of phosphoarginine, but a pH-buffering effect cannot be excluded.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1023/a:1024172518062
- PMID 12889807
- Persistent URL
- https://sonar.ch/global/documents/47096
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