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Hydrogen/deuterium isotope effect in the oscillating peroxidase-oxidase reaction.
Journal article

Hydrogen/deuterium isotope effect in the oscillating peroxidase-oxidase reaction.

  • Rys P Laboratorium für Technische Chemie, Eidgenössische Technische Hochschule (ETH), Zurich, Switzerland.
  • Wang J
  • 1992-07-31
Published in:
  • Biochemical and biophysical research communications. - 1992
Deuterium
Horseradish Peroxidase
Hydrogen
Isotope Labeling
Kinetics
NAD
Oxidation-Reduction
Spectrophotometry
Time Factors
English The oscillatory peroxidase-oxidase reaction has been investigated by using NADH deuterated in the nicotinamide 4-A position. A considerable kinetic hydrogen/deuterium isotope effect on the oscillatory behavior was revealed, which may provide an additional valuable tool for mechanistic studies and for discriminating between various mechanistic models of the peroxidase-oxidase reaction. Particularly, this effect manifests in different oscillation frequencies. A sequence of simple and aperiodic oscillations was found between two stable steady states.
Language
  • English
Open access status
closed
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Persistent URL
https://sonar.ch/global/documents/47371
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