Hydrogen/deuterium isotope effect in the oscillating peroxidase-oxidase reaction.

Rys P; Wang J

doi:10.1016/0006-291x(92)90790-r

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Hydrogen/deuterium isotope effect in the oscillating peroxidase-oxidase reaction.

Rys P Laboratorium für Technische Chemie, Eidgenössische Technische Hochschule (ETH), Zurich, Switzerland.
Wang J

1992-07-31

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Biochemical and biophysical research communications. - 1992

English The oscillatory peroxidase-oxidase reaction has been investigated by using NADH deuterated in the nicotinamide 4-A position. A considerable kinetic hydrogen/deuterium isotope effect on the oscillatory behavior was revealed, which may provide an additional valuable tool for mechanistic studies and for discriminating between various mechanistic models of the peroxidase-oxidase reaction. Particularly, this effect manifests in different oscillation frequencies. A sequence of simple and aperiodic oscillations was found between two stable steady states.

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