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[Determination of biogenic amines by RP-HPLC for monitoring the microbial spoilage of poultry].
Journal article

[Determination of biogenic amines by RP-HPLC for monitoring the microbial spoilage of poultry].

  • 1988-08-01
Published in:
  • Zeitschrift fur Lebensmittel-Untersuchung und -Forschung. - 1988
Animals
Biogenic Amines
Cadaverine
Chickens
Chromatography, High Pressure Liquid
Food Contamination
Food Microbiology
Indicators and Reagents
Meat
Putrescine
Spectrophotometry, Ultraviolet
English A simple high-performance liquid chromatographic (HPLC) analysis is described for the determination of biogenic amines in broiler carcasses. The clean-up procedure consists of an extraction with 0.6 M-perchloric acid, formation of dansyl derivatives, separation by a RP-18 column and UV detection at 254 nm. Within 7 min eight amines could be separated. The quantitative determination of spermidine and spermine requires an additional ion-exchange clean-up with Amberlite CG 50 after the extraction. This procedure gives recoveries of 82%-96% with detection limits of 0.2-0.5 microgram/g of broiler skin. Putrescine and cadaverine are good indicators for the onset of spoilage of poultry carcasses, since both amines could be detected at total colony counts of 10(5) cfu/cm2 and their concentration increases rapidly with advancing decomposition.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/47394
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