Purification of two isofunctional hydrolases (EC 3.7.1.8) in the degradative pathway for dibenzofuran in Sphingomonas sp. strain RW1.
- Bünz PV Institute of Microbiology, ETH-Zentrum, Zürich, Switzerland.
- Falchetto R
- Cook AM
- 1993-01-01
Published in:
- Biodegradation. - 1993
Bacteroidetes
Base Sequence
Benzoates
Benzofurans
Benzoic Acid
Biodegradation, Environmental
Catechols
Fatty Acids, Unsaturated
Hydrolases
Isoenzymes
Molecular Sequence Data
Oxygenases
Salicylates
Salicylic Acid
English
Sphingomonas sp. strain RW1, when grown in salicylate-salts medium, synthesized the enzymes for the degradation of dibenzofuran. The reaction subsequent to meta cleavage of the first benzene ring was found to be catalyzed by two isofunctional hydrolases, H1 and H2, which were purified by chromatography on anion exchange, hydrophobic interaction and gel filtration media. Each enzyme was able to hydrolyze 2-hydroxy-6-oxo-6-(2-hydroxyphenyl)hexa-2,4-dienoate and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate to produce salicylate and benzoate, respectively. SDS/PAGE of each purified enzyme showed a single band of M(r) 31,000 (H1) or 29,000 (H2). The N-terminal amino acid sequences of the two proteins showed 50% homology.
- Language
-
- English
- Open access status
- green
- Identifiers
-
- DOI 10.1007/bf00695119
- PMID 7764304
- Persistent URL
- https://sonar.ch/global/documents/47402
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