HOPX Defines Heterogeneity of Postnatal Subventricular Zone Neural Stem Cells.
- Zweifel S Université de Lyon, Université Claude Bernard Lyon 1, INSERM, Stem Cell and Brain Research Institute U1208, Bron 69500, France.
- Marcy G Université de Lyon, Université Claude Bernard Lyon 1, INSERM, Stem Cell and Brain Research Institute U1208, Bron 69500, France; Ecole Pratique des Hautes Etudes, PSL Research University, Neurogenetics Department, Paris 75014, France.
- Lo Guidice Q Université de Lyon, Université Claude Bernard Lyon 1, INSERM, Stem Cell and Brain Research Institute U1208, Bron 69500, France.
- Li D Department of Cell and Developmental Biology, Cardiovascular Institute, Institute for Regenerative Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19104, USA.
- Heinrich C Université de Lyon, Université Claude Bernard Lyon 1, INSERM, Stem Cell and Brain Research Institute U1208, Bron 69500, France.
- Azim K Brain Research Institute, University of Zurich, Zurich 8057, Switzerland. Electronic address: kasum.azim@med.uni-duesseldorf.de.
- Raineteau O Université de Lyon, Université Claude Bernard Lyon 1, INSERM, Stem Cell and Brain Research Institute U1208, Bron 69500, France. Electronic address: olivier.raineteau@inserm.fr.
- 2018-09-04
Published in:
- Stem cell reports. - 2018
gliogenesis
neurogenesis
outer radial glia
postnatal
radial glia
regionalization
subventricular zone
transcriptional heterogeneity
Animals
Astrocytes
Cell Lineage
Gene Expression Regulation, Developmental
Homeodomain Proteins
Lateral Ventricles
Mice
Mice, Inbred C57BL
Neural Stem Cells
Neurogenesis
Neuroglia
Transcriptome
English
The largest diversity of neural lineages generated from the subventricular zone (SVZ) occurs early after birth and is regulated in a spatiotemporal manner depending on the expression of specific transcriptional cues. Transcriptomics and fate-mapping approaches were employed to explore the relationship between regional expression of transcription factors by neural stem cells (NSCs) and the specification of distinct neural lineages. Our results support an early priming of NSCs for the genesis of defined cell types depending on their spatial location in the SVZ and identify HOPX as a marker of a subpopulation primed toward astrocytic fates. Manipulation of HOPX expression, however, showed no effect on astrogenesis but resulted in marked changes in the number of NSCs and of their progenies. Taken together, our results highlight transcriptional and spatial heterogeneity of postnatal NSCs and reveal a key role for HOPX in controlling SVZ germinal activity.
- Language
-
- English
- Open access status
- gold
- Identifiers
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- DOI 10.1016/j.stemcr.2018.08.006
- PMID 30174314
- Persistent URL
- https://sonar.ch/global/documents/47414
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