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The dynamics of both filamentous and globular mammalian reovirus viral factories rely on the microtubule network.
Journal article

The dynamics of both filamentous and globular mammalian reovirus viral factories rely on the microtubule network.

  • Eichwald C Institute of Virology, University of Zurich, Winterthurerstrasse 266a, 8057 Zurich, Switzerland; Dept. of Molecular Genetics and Immunobiology, Harvard Medical School, Harvard University, 77 Av. Louis Pasteur, Boston, MA 02115, USA. Electronic address: ceichwald@vetvir.uzh.ch.
  • Ackermann M Institute of Virology, University of Zurich, Winterthurerstrasse 266a, 8057 Zurich, Switzerland. Electronic address: mathias.ackermann@uzh.ch.
  • Nibert ML Dept. of Molecular Genetics and Immunobiology, Harvard Medical School, Harvard University, 77 Av. Louis Pasteur, Boston, MA 02115, USA. Electronic address: max_nibert@hms.harvard.edu.
  • 2018-02-19
Published in:
  • Virology. - 2018
Acetylated-tubulin
Coalescence
Dynamic
Dynein
Microtubules
Perinuclear
Reoviridae
Viral factories
µ2
µNS
Animals
Cell Line
Gene Expression Regulation, Viral
Microtubules
Molecular Motor Proteins
Orthoreovirus, Mammalian
Virus Replication
English Mammalian reovirus viral factories (VFs) form filamentous or globular structures depending on the viral strain. In this study, we attempt to characterize the dynamics of both filamentous and globular VFs. Here, we present evidence demonstrating that globular VFs are dynamic entities coalescing between them, thereby gaining in size and concomitantly decreasing in numbers during the course of the infection. Additionally, both kinds of VFs condense into a perinuclear position. Our results show that globular VFs rely on an intact MT-network for dynamic motion, structural assembly, and maintenance and for perinuclear condensation. Interestingly, dynein localizes in both kinds of VFs, having a role at least in large globular VFs formation. To study filamentous VF dynamics, we used different transfection ratios of µNS with filamentous µ2. We found a MT-network dependency for VF-like structures perinuclear condensation. Also, µNS promotes VFLSs perinuclear positioning as well as an increase in acetylated tubulin levels.
Language
  • English
Open access status
bronze
Identifiers
Persistent URL
https://sonar.ch/global/documents/47471
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