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Probing the canonicity of the Wnt/Wingless signaling pathway.

  • Franz A Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.
  • Shlyueva D Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), Campus-Vienna-Biocenter 1, Vienna, Austria.
  • Brunner E Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.
  • Stark A Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), Campus-Vienna-Biocenter 1, Vienna, Austria.
  • Basler K Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.
  • 2017-04-04
Published in:
  • PLoS genetics. - 2017
Animals
Armadillo Domain Proteins
Drosophila Proteins
Drosophila melanogaster
Enhancer Elements, Genetic
Gene Expression Regulation
Genome, Insect
Repressor Proteins
Sequence Analysis, RNA
Signal Transduction
Transcription Factors
Wnt Signaling Pathway
Wnt1 Protein
English The hallmark of canonical Wnt signaling is the transcriptional induction of Wnt target genes by the beta-catenin/TCF complex. Several studies have proposed alternative interaction partners for beta-catenin or TCF, but the relevance of potential bifurcations in the distal Wnt pathway remains unclear. Here we study on a genome-wide scale the requirement for Armadillo (Arm, Drosophila beta-catenin) and Pangolin (Pan, Drosophila TCF) in the Wnt/Wingless(Wg)-induced transcriptional response of Drosophila Kc cells. Using somatic genetics, we demonstrate that both Arm and Pan are absolutely required for mediating activation and repression of target genes. Furthermore, by means of STARR-sequencing we identified Wnt/Wg-responsive enhancer elements and found that all responsive enhancers depend on Pan. Together, our results confirm the dogma of canonical Wnt/Wg signaling and argue against the existence of distal pathway branches in this system.
Language
  • English
Open access status
gold
Identifiers
Persistent URL
https://sonar.ch/global/documents/47523
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