Structural basis for pH-dependent retrieval of ER proteins from the Golgi by the KDEL receptor.

Bräuer P; Parker JL; Gerondopoulos A; Zimmermann I; Seeger MA; Barr FA; Newstead S

doi:10.1126/science.aaw2859

Back

Journal article

Structural basis for pH-dependent retrieval of ER proteins from the Golgi by the KDEL receptor.

Bräuer P Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
Parker JL Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
Gerondopoulos A Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
Zimmermann I Institute of Medical Microbiology, University of Zurich, 8006 Zurich, Switzerland.
Seeger MA Institute of Medical Microbiology, University of Zurich, 8006 Zurich, Switzerland.
Barr FA Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK. simon.newstead@bioch.ox.ac.uk francis.barr@bioch.ox.ac.uk.
Newstead S Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK. simon.newstead@bioch.ox.ac.uk francis.barr@bioch.ox.ac.uk.

2019-03-09

Published in:

Science (New York, N.Y.). - 2019

English Selective export and retrieval of proteins between the endoplasmic reticulum (ER) and Golgi apparatus is indispensable for eukaryotic cell function. An essential step in the retrieval of ER luminal proteins from the Golgi is the pH-dependent recognition of a carboxyl-terminal Lys-Asp-Glu-Leu (KDEL) signal by the KDEL receptor. Here, we present crystal structures of the chicken KDEL receptor in the apo ER state, KDEL-bound Golgi state, and in complex with an antagonistic synthetic nanobody (sybody). These structures show a transporter-like architecture that undergoes conformational changes upon KDEL binding and reveal a pH-dependent interaction network crucial for recognition of the carboxyl terminus of the KDEL signal. Complementary in vitro binding and in vivo cell localization data explain how these features create a pH-dependent retrieval system in the secretory pathway.

Language