SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy.
- Lukinavičius G Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, NCCR in Chemical Biology, 1015 Lausanne, Switzerland.
- Blaukopf C Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter Campus (VBC), Dr Bohr Gasse 3, 1030 Vienna, Austria.
- Pershagen E Department of Chemistry-BMC, Uppsala University, 75123 Uppsala, Sweden.
- Schena A Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, NCCR in Chemical Biology, 1015 Lausanne, Switzerland.
- Reymond L Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, NCCR in Chemical Biology, 1015 Lausanne, Switzerland.
- Derivery E Department of Biochemistry, NCCR in Chemical Biology, University of Geneva, 1211 Geneva, Switzerland.
- Gonzalez-Gaitan M Department of Biochemistry, NCCR in Chemical Biology, University of Geneva, 1211 Geneva, Switzerland.
- D'Este E Department NanoBiophotonics, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.
- Hell SW Department NanoBiophotonics, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.
- Wolfram Gerlich D Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter Campus (VBC), Dr Bohr Gasse 3, 1030 Vienna, Austria.
- Johnsson K Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, NCCR in Chemical Biology, 1015 Lausanne, Switzerland.
- 2015-10-02
Published in:
- Nature communications. - 2015
Animals
Cell Line
DNA
Drosophila
Fluorescent Dyes
Humans
Microscopy
Microscopy, Fluorescence
Molecular Imaging
Molecular Structure
Staining and Labeling
English
Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA stain, SiR-Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy. The combination of these properties makes this probe a powerful tool for live-cell imaging.
- Language
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- English
- Open access status
- gold
- Identifiers
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- DOI 10.1038/ncomms9497
- PMID 26423723
- Persistent URL
- https://sonar.ch/global/documents/50325
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