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Self-establishing communities enable cooperative metabolite exchange in a eukaryote.
Journal article

Self-establishing communities enable cooperative metabolite exchange in a eukaryote.

  • Campbell K Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom.
  • Vowinckel J Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom.
  • Mülleder M Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom.
  • Malmsheimer S Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom.
  • Lawrence N The Wellcome Trust Gurdon Institute, University of Cambridge, Cambridge, United Kingdom.
  • Calvani E Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom.
  • Miller-Fleming L Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom.
  • Alam MT Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom.
  • Christen S Institute of Molecular Systems Biology, ETH Zürich, Zurich, Switzerland.
  • Keller MA Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom.
  • Ralser M Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom.
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  • 2015-10-27
Published in:
  • eLife. - 2015
cell biology
cellular heterogeneity
computational biology
cooperativity
metabolism
s.cerevisae
systems biology
Amino Acids
Coculture Techniques
Metabolome
Microbial Interactions
Microbial Viability
Saccharomyces cerevisiae
English Metabolite exchange among co-growing cells is frequent by nature, however, is not necessarily occurring at growth-relevant quantities indicative of non-cell-autonomous metabolic function. Complementary auxotrophs of Saccharomyces cerevisiae amino acid and nucleotide metabolism regularly fail to compensate for each other's deficiencies upon co-culturing, a situation which implied the absence of growth-relevant metabolite exchange interactions. Contrastingly, we find that yeast colonies maintain a rich exometabolome and that cells prefer the uptake of extracellular metabolites over self-synthesis, indicators of ongoing metabolite exchange. We conceived a system that circumvents co-culturing and begins with a self-supporting cell that grows autonomously into a heterogeneous community, only able to survive by exchanging histidine, leucine, uracil, and methionine. Compensating for the progressive loss of prototrophy, self-establishing communities successfully obtained an auxotrophic composition in a nutrition-dependent manner, maintaining a wild-type like exometabolome, growth parameters, and cell viability. Yeast, as a eukaryotic model, thus possesses extensive capacity for growth-relevant metabolite exchange and readily cooperates in metabolism within progressively establishing communities.
Language
  • English
Open access status
gold
Identifiers
Persistent URL
https://sonar.ch/global/documents/52435
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