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Antigen array for serological diagnosis and novel allergen identification in severe equine asthma.

  • White SJ Royal Agricultural University, Cirencester, Gloucestershire, GL7 6JS, UK. samuel.white@ntu.ac.uk.
  • Moore-Colyer M Royal Agricultural University, Cirencester, Gloucestershire, GL7 6JS, UK.
  • Marti E Department of Clinical Research and Veterinary Public Health, University of Bern, Bremgartenstr, Postfach, 3001, Bern, Switzerland.
  • Hannant D School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK.
  • Gerber V Department of Clinical Research and Veterinary Public Health, University of Bern, Bremgartenstr, Postfach, 3001, Bern, Switzerland.
  • Coüetil L Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, IN, 47907, USA.
  • Richard EA LABÉO Frank Duncombe, 1 route de Rosel, 14053, Caen, Cedex 4, France.
  • Alcocer M School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK.
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  • 2019-10-25
Published in:
  • Scientific reports. - 2019
Allergens
Animals
Antigens
Asthma
Discriminant Analysis
Horses
Least-Squares Analysis
Serologic Tests
English Severe equine asthma (sEA), which closely resembles human asthma, is a debilitating and performance-limiting allergic respiratory disorder which affects 14% of horses in the Northern Hemisphere and is associated with increased allergen-specific immunoglobulin E (IgE) against a range of environmental proteins. A comprehensive microarray platform was developed to enable the simultaneous detection of allergen-specific equine IgE in serum against a wide range of putative allergenic proteins. The microarray revealed a plethora of novel pollen, bacteria, mould and arthropod proteins significant in the aetiology of sEA. Moreover, the analyses revealed an association between sEA-affected horses and IgE antibodies specific for proteins derived from latex, which has traditionally been ubiquitous to the horse's environment in the form of riding surfaces and race tracks. Further work is required to establish the involvement of latex proteins in sEA as a potential risk factor. This work demonstrates a novel and rapid approach to sEA diagnosis, providing a platform for tailored management and the development of allergen-specific immunotherapy.
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  • English
Open access status
gold
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https://sonar.ch/global/documents/52909
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