Reproduction of contagious bovine pleuropneumonia via aerosol-based challenge with Mycoplasma mycoides subsp. mycoides.
- Sacchini F International Livestock Research Institute, Old Naivasha Road, PO Box 30709, Nairobi, KE-00100, Kenya.
- Liljander AM International Livestock Research Institute, Old Naivasha Road, PO Box 30709, Nairobi, KE-00100, Kenya.
- Heller M Friedrich-Loeffler-Institute-Federal Research Institute for Animal Health, Naumburger Str. 96a, 07743, Jena, Germany.
- Poole EJ International Livestock Research Institute, Old Naivasha Road, PO Box 30709, Nairobi, KE-00100, Kenya.
- Posthaus H Institute of Animal Pathology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Längassstrasse 122, CH-3012, Bern, Switzerland.
- Schieck E International Livestock Research Institute, Old Naivasha Road, PO Box 30709, Nairobi, KE-00100, Kenya.
- Jores J International Livestock Research Institute, Old Naivasha Road, PO Box 30709, Nairobi, KE-00100, Kenya. joerg.jores@vetsuisse.unibe.ch.
- 2020-11-17
Published in:
- Acta veterinaria Scandinavica. - 2020
Aerosol
CBPP
Contagious bovine pleuropneumonia
Infection model
Intranasal
Mycoplasma mycoides subsp. mycoides
Spray
English
Contagious bovine pleuropneumonia (CBPP) is a respiratory disease caused by Mycoplasma mycoides subsp. mycoides. Infection occurs via Mycoplasma-containing droplets and therefore requires close contact between animals. The current infection models are suboptimal and based on intratracheal installation of mycoplasmas or in-contact infection. This work tested the infection of adult cattle via aerosols containing live mycoplasmas mimicking the infection of cattle in the field. Therefore, we infected six cattle with aerosolized Mycoplasma mycoides subsp. mycoides strain Afadé over seven consecutive days with altogether 109 colony forming units. All animals seroconverted between 11-24 days post infection and five out of six animals showed typical CBPP lesions. One animal did not show any lung lesions at necropsy, while another animal had to be euthanized at 25 days post infection because it reached endpoint criteria. Seroconversion confirmed successful infection and the spectrum of clinical and lesions observed mirrors epidemiological models and the field situation, in which only a fraction of animals suffers from acute clinical disease post infection.
- Language
-
- English
- Open access status
- gold
- Identifiers
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- DOI 10.1186/s13028-020-00560-0
- PMID 33198794
- Persistent URL
- https://sonar.ch/global/documents/53027
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