DNA unwinding activity of replication protein A.
- Georgaki A Department of Pharmacology and Biochemistry, University of Zürich-Irchel, Switzerland.
- Strack B
- Podust V
- Hübscher U
- 1992-08-24
Published in:
- FEBS letters. - 1992
Adenoviridae
DNA Helicases
DNA Replication
DNA-Binding Proteins
Escherichia coli
Magnesium Chloride
Replication Protein A
Sodium Chloride
T-Phages
Thymus Gland
English
Replication protein A (RP-A) is a heterotrimeric complex conserved in eukaryotic cells. It binds to single-stranded DNA and is essential for initiation and elongation of DNA replication. In this communication we give evidence that this protein can unwind DNA independent of magnesium and ATP, two essential cofactors for bona fide DNA helicase activity. RP-A can unwind up to at least 350 basepairs and appears to be required in stoichiometric amounts. The reaction is extremely sensitive to NaCl and MgCl2. This activity of RF-A is suggestive for a possible unwinding function in initiation of DNA replication in eukaryotes.
- Language
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- English
- Open access status
- bronze
- Identifiers
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- DOI 10.1016/0014-5793(92)81283-r
- PMID 1324186
- Persistent URL
- https://sonar.ch/global/documents/55229
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