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ITP in dynamically double-coated fused-silica capillaries.
Journal article

ITP in dynamically double-coated fused-silica capillaries.

  • 2006-12-01
Published in:
  • Electrophoresis. - 2006
Acids
Computer Simulation
Electrophoresis, Capillary
Hexadimethrine Bromide
Hydrogen-Ion Concentration
Models, Chemical
Osmosis
Polyvinyls
Silicon Dioxide
Sulfonic Acids
English Bidirectional ITP in fused-silica capillaries double-coated with Polybrene and poly-(vinylsulfonate) is a robust approach for analysis of low-molecular-mass compounds. EOF towards the cathode is strong (mobility >4.0 x 10(-8) m(2)/Vs) within the entire pH range investigated (2.40-8.08), dependent on ionic strength and buffer used and, at constant ionic strength, higher at alkaline pH. Electrokinetic separations and transport in such coated capillaries can be described with a dynamic computer model which permits the combined simulation of electrophoresis and electroosmosis in which the EOF is predicted either with a constant (i.e. pH- and ionic strength-independent) or a pH- and ionic strength-dependent electroosmotic mobility. Detector profiles predicted by computer simulation agree qualitatively well with bidirectional isotachopherograms that are monitored with a setup comprising two axial contactless conductivity detectors and a UV absorbance detector. The varying EOF predicted with a pH- and ionic strength-dependent electroosmotic mobility can be regarded as being realistic.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/56012
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