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Vascular Morphodynamics During Secondary Growth.
Journal article

Vascular Morphodynamics During Secondary Growth.

  • de Reuille PB University of Bern, Altenbergrain 21, 3013, Bern, Switzerland.
  • Ragni L Center for Plant Molecular Biology-ZMBP, Developmental Genetics, University of Tübingen, Auf der Morgenstelle 32, 72076, Tübingen, Germany. laura.ragni@zmbp.uni-tuebingen.de.
  • 2017-01-05
Published in:
  • Methods in molecular biology (Clifton, N.J.). - 2017
Arabidopsis hypocotyl
Cell segmentation
LithoGraphX
Quantitative histology
Arabidopsis
Image Interpretation, Computer-Assisted
Image Processing, Computer-Assisted
Microscopy
Plant Development
Plant Vascular Bundle
Software
English Quantification of vascular morphodynamics during secondary growth has been hampered by the scale of the process. Even in the tiny model plant Arabidopsis thaliana, the xylem can include more than 2000 cells in a single cross section, rendering manual counting impractical. Moreover, due to its deep location, xylem is an inaccessible tissue, limiting live imaging. A novel method to visualize and measure secondary growth progression has been proposed: "the Quantitative Histology" approach. This method is based on a detailed anatomical atlas, and image segmentation coupled with machine learning to automatically extract cell shapes and identify cell type. Here we present a new version of this approach, with a user-friendly interface implemented in the open source software LithoGraphX.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/58043
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