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Urinary zearalenone measured with ELISA as a biomarker of zearalenone exposure in pigs.
Journal article

Urinary zearalenone measured with ELISA as a biomarker of zearalenone exposure in pigs.

  • 2014-05-30
Published in:
  • Mycotoxin research. - 2014
Animals
Biomarkers
Enzyme-Linked Immunosorbent Assay
Swine
Urine
Zearalenone
English The suitability to assess zearalenone (ZEA) exposure in pigs of a commercial ELISA kit for ZEA analysis in urine was tested. A daily dose of 0, 5, 10, 20 and 40 μg synthetic ZEA per kilogram BW was administered via the feed to four gilts per dose group, and after 3 and after 7 days of ZEA intake, urine samples were assayed with the ELISA which has a relative cross-reactivity of 42% with α-zearalenol. The concentration of urinary ZEA equivalents (ZEA plus 42% of α-zearalenol present) did not differ between day 4 and day 8 (P = 0.50) within each dose group. The urinary ZEA equivalent/creatinine ratio was tightly correlated with ZEA intake (r = 0.95). The urinary ZEA equivalent/creatinine values at 0 and 40 μg/kg BW were distinctly different from those of the intermediate dose levels, whereas there was some overlapping of the individual values at the dose levels 5, 10 and 20 μg/kg BW. The urinary ZEA equivalent/creatinine ratio can be used as a biomarker for ZEA exposure in pigs provided that urine samples of several animals receiving the same diet are assayed, either separately or after pooling.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/58568
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