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Colchicine-induced stimulation of PMN motility related to cytoskeletal changes in actin, alpha-actinin, and myosin.
Journal article

Colchicine-induced stimulation of PMN motility related to cytoskeletal changes in actin, alpha-actinin, and myosin.

  • 1993-01-01
Published in:
  • Cell motility and the cytoskeleton. - 1993
Actinin
Actins
Cell Movement
Colchicine
Cytoskeleton
Humans
In Vitro Techniques
Myosins
Neutrophils
Tubulin
English Colchicine-induced stimulation of polymorphonuclear leukocyte (PMN) locomotion is an interesting model because extension of blebs at the front occurs at a rate (about 2.4 microns/s) which is far above that reported for growth of actin filaments. The following cytoskeletal changes were observed in colchicine-treated PMNs: (1) a small increase in cytoskeleton-associated actin was noted, as well as a somewhat more pronounced increase in cytoskeleton-associated alpha-actinin, as compared with untreated or DMSO-treated controls. There was, however, no measurable increase in F-actin as determined by NBD-phallacidin binding; (2) the values for the ratio (alpha-actinin/actin) are lower in PMNs treated with colchicine for 30 min, as compared with PMNs stimulated with fNLPNTL for 1 minute (non-polar ruffling cells) or 30 min (polarized locomoting cells); thus, this ratio may depend on the type of PMN motility; (3) in polarized PMNs F-actin was mainly located linearly all along the cell membrane; there was more intense staining at the front of the cells; (4) alpha-actinin appeared to colocalize with F-actin at the leading front, but not with F-actin at the tail of polarized cells; (5) myosin was preferentially found at the rear part of polarized cells but not or only to a small extent at the front. Our data indicate a close functional correlation between microtubules and microfilaments. We speculate that F-actin in combination with alpha-actinin promotes expansion of pseudopods, whereas myosin combined with F-actin promotes contraction.(ABSTRACT TRUNCATED AT 250 WORDS)
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/59799
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