DNA extraction from Ascaris suum muscle tissue.
- Di Mito C Parasitologie, Institut de zoologie, Université de Neuchâtel, Switzerland.
- Betschart B
- 1998-08-07
Published in:
- Parasitology research. - 1998
Animals
Ascaris suum
Cetrimonium
Cetrimonium Compounds
DNA, Helminth
Deoxyribonuclease EcoRI
Electrophoresis, Agar Gel
Muscles
Parasitology
Polymerase Chain Reaction
Povidone
English
A new method for the extraction of DNA from Ascaris suum muscle has been developed. It combines a standard SDS-based extraction with a plant DNA extraction procedure. The use of SDS and proteinase K allows the elimination of proteins, while CTAB and polyclar AT eliminate glycogen and polyphenols. The DNA thus obtained can easily be digested by endonucleases and amplified by PCR.
- Language
-
- English
- Open access status
- green
- Identifiers
-
- DOI 10.1007/s004360050456
- PMID 9694380
- Persistent URL
- https://sonar.ch/global/documents/599
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