Pathotyping Escherichia coli by Using Miniaturized DNA Microarrays

Anjum, Muna F.; Mafura, Muriel; Slickers, Peter; Ballmer, Karin; Kuhnert, Peter; Woodward, Martin J.; Ehricht, Ralf

doi:10.1128/aem.00419-07

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Journal article

Pathotyping Escherichia coli by Using Miniaturized DNA Microarrays

Anjum, Muna F. Department of Food and Environmental Safety, Veterinary Laboratories Agency-Weybridge, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom
Mafura, Muriel Department of Food and Environmental Safety, Veterinary Laboratories Agency-Weybridge, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom
Slickers, Peter CLONDIAG Chip Technologies GmbH, Jena, Germany
Ballmer, Karin Institute of Veterinary Bacteriology, Vetsuisse Faculty of the University of Bern, Bern, Switzerland
Kuhnert, Peter Institute of Veterinary Bacteriology, Vetsuisse Faculty of the University of Bern, Bern, Switzerland
Woodward, Martin J. Department of Food and Environmental Safety, Veterinary Laboratories Agency-Weybridge, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom
Ehricht, Ralf CLONDIAG Chip Technologies GmbH, Jena, Germany

Published in:

Applied and Environmental Microbiology. - American Society for Microbiology. - 2007, vol. 73, no. 17, p. 5692-5697

Applied Microbiology and Biotechnology

English ABSTRACT
The detection of virulence determinants harbored by pathogenic Escherichia coli is important for establishing the pathotype responsible for infection. A sensitive and specific miniaturized virulence microarray containing 60 oligonucleotide probes was developed. It detected six E. coli pathotypes and will be suitable in the future for high-throughput use.

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