Single-Cell RNA Sequencing of Lymph Node Stromal Cells Reveals Niche-Associated Heterogeneity.
- Rodda LB Howard Hughes Medical Institute and Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA.
- Lu E Howard Hughes Medical Institute and Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA.
- Bennett ML Department of Neurobiology, Stanford University School of Medicine, Stanford, CA 94305, USA.
- Sokol CL Center for Immunology & Inflammatory Diseases, Division of Rheumatology, Allergy & Immunology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
- Wang X Department of Immunology, Nanjing Medical University, Nanjing, China.
- Luther SA Department of Biochemistry, Center for Immunity and Infection, University of Lausanne, 1066 Epalinges, Switzerland.
- Barres BA Department of Neurobiology, Stanford University School of Medicine, Stanford, CA 94305, USA.
- Luster AD Center for Immunology & Inflammatory Diseases, Division of Rheumatology, Allergy & Immunology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
- Ye CJ Institute for Human Genetics, Department of Epidemiology and Biostatistics, Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, CA 94143, USA.
- Cyster JG Howard Hughes Medical Institute and Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA. Electronic address: jason.cyster@ucsf.edu.
- 2018-05-13
Published in:
- Immunity. - 2018
double negative cell
fibroblastic reticular cell
follicular dendritic cell
marginal reticular cell
perivascular cell
single-cell RNA sequencing
stromal cell
Animals
Chemokine CCL19
Dendritic Cells, Follicular
Female
Lymph Nodes
Lymphoid Tissue
Mice, Inbred C57BL
Sequence Analysis, RNA
Single-Cell Analysis
Stromal Cells
Transcriptome
English
Stromal cells (SCs) establish the compartmentalization of lymphoid tissues critical to the immune response. However, the full diversity of lymph node (LN) SCs remains undefined. Using droplet-based single-cell RNA sequencing, we identified nine peripheral LN non-endothelial SC clusters. Included are the established subsets, Ccl19hi T-zone reticular cells (TRCs), marginal reticular cells, follicular dendritic cells (FDCs), and perivascular cells. We also identified Ccl19lo TRCs, likely including cholesterol-25-hydroxylase+ cells located at the T-zone perimeter, Cxcl9+ TRCs in the T-zone and interfollicular region, CD34+ SCs in the capsule and medullary vessel adventitia, indolethylamine N-methyltransferase+ SCs in the medullary cords, and Nr4a1+ SCs in several niches. These data help define how transcriptionally distinct LN SCs support niche-restricted immune functions and provide evidence that many SCs are in an activated state.
- Language
-
- English
- Open access status
- bronze
- Identifiers
-
- DOI 10.1016/j.immuni.2018.04.006
- PMID 29752062
- Persistent URL
- https://sonar.ch/global/documents/607
Statistics
Document views: 60
File downloads:
- fulltext.pdf: 0