Journal article

Genetic and Phenotypic Characterization of Cryphonectria hypovirus 1 from Eurasian Georgia.

  • Rigling D WSL Swiss Federal Research Institute, Zürcherstrasse 111, 8903 Birmensdorf; Switzerland. daniel.rigling@wsl.ch.
  • Borst N WSL Swiss Federal Research Institute, Zürcherstrasse 111, 8903 Birmensdorf; Switzerland. nora@breuert.eu.
  • Cornejo C WSL Swiss Federal Research Institute, Zürcherstrasse 111, 8903 Birmensdorf; Switzerland. carolina.cornejo@wsl.ch.
  • Supatashvili A Vasil Gulisashvili Forestry Institute, Agricultural University of Georgia, 0186 Tbilisi, Georgia. archil.tbilisi@hotmail.com.
  • Prospero S WSL Swiss Federal Research Institute, Zürcherstrasse 111, 8903 Birmensdorf; Switzerland. simone.prospero@wsl.ch.
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  • 2018-12-06
Published in:
  • Viruses. - 2018
English Cryphonectria hypovirus 1 (CHV-1) infects the chestnut blight fungus Cryphonectria parasitica and acts as a biological control agent against this harmful tree disease. In this study, we screened the recently characterized C. parasitica population in Eurasian Georgia for the presence of CHV-1. We found 62 CHV-1 infected C. parasitica isolates (9.3%) among a total of 664 isolates sampled in 14 locations across Georgia. The prevalence of CHV-1 at the different locations ranged from 0% in the eastern part of the country to 29% in the western part. Sequencing of two specific regions of the viral genome one each in ORFA and ORFB revealed a unique CHV-1 subtype in Georgia. This subtype has a recombinant pattern combining the ORFA region from the subtype F2 and the ORFB region from subtype D. All 62 viral strains belonged to this Georgian CHV-1 subtype (subtype G). The CHV-1 subtype G strongly reduced the parasitic growth of C. parasitica isolates from Georgia, with a more severe effect on the European genepool compared to the Georgian genepool. The CHV-1 subtype detected in Georgia provides a valuable candidate for biological control applications in the Caucasus region.
Language
  • English
Open access status
gold
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Persistent URL
https://sonar.ch/global/documents/61465
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