Effect of tolytoxin on tunneling nanotube formation and function.
- Dilsizoglu Senol A Institut Pasteur, Unité Trafic Membranaire et Pathogenèse, 25-28 Rue du Docteur Roux, 75724, Paris CEDEX 15, France.
- Pepe A Institut Pasteur, Unité Trafic Membranaire et Pathogenèse, 25-28 Rue du Docteur Roux, 75724, Paris CEDEX 15, France.
- Grudina C Institut Pasteur, Unité Trafic Membranaire et Pathogenèse, 25-28 Rue du Docteur Roux, 75724, Paris CEDEX 15, France.
- Sassoon N Institut Pasteur, Collection des Cyanobactéries, 25-28 Rue du Docteur Roux, 75724, Paris CEDEX 15, France.
- Reiko U Institute of Microbiology, Eigenössische Technische Hochschule (ETH) Zurich, Vladimir-Prelog-Weg 4, 8093, Zurich, Switzerland.
- Bousset L Paris-Saclay Institute of Neuroscience, CNRS, 1 Avenue de la Terrasse, 91190, Gif-sur-Yvette, France.
- Melki R Paris-Saclay Institute of Neuroscience, CNRS, 1 Avenue de la Terrasse, 91190, Gif-sur-Yvette, France.
- Piel J Institute of Microbiology, Eigenössische Technische Hochschule (ETH) Zurich, Vladimir-Prelog-Weg 4, 8093, Zurich, Switzerland.
- Gugger M Institut Pasteur, Collection des Cyanobactéries, 25-28 Rue du Docteur Roux, 75724, Paris CEDEX 15, France. muriel.gugger@pasteur.fr.
- Zurzolo C Institut Pasteur, Unité Trafic Membranaire et Pathogenèse, 25-28 Rue du Docteur Roux, 75724, Paris CEDEX 15, France. chiara.zurzolo@pasteur.fr.
- 2019-04-07
Published in:
- Scientific reports. - 2019
English
Tunneling nanotubes (TNTs) are actin-containing membrane protrusions that play an essential role in long-range intercellular communication. They are involved in development of various diseases by allowing transfer of pathogens or protein aggregates as well as organelles such as mitochondria. Increase in TNT formation has been linked to many pathological conditions. Here we show that nM concentrations of tolytoxin, a cyanobacterial macrolide that targets actin by inhibition of its polymerization, significantly decrease the number of TNT-connected cells, as well as transfer of mitochondria and α-synuclein fibrils in two different cell lines of neuronal (SH-SY5Y) and epithelial (SW13) origin. As the cytoskeleton of the tested cell remain preserved, this macrolide could serve as a valuable tool for future therapies against diseases propagated by TNTs.
- Language
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- English
- Open access status
- gold
- Identifiers
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- DOI 10.1038/s41598-019-42161-6
- PMID 30952909
- Persistent URL
- https://sonar.ch/global/documents/61552
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