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Rationally modified estrogen receptor protein as a bio-recognition element for the detection of EDC pollutants: strategies and opportunities.

  • Pedotti M Institute for Research in Biomedicine, Universita' della Svizzera italiana (USI) via Vela 6, 6500 Bellinzona, Switzerland. mattia.pedotti@irb.usi.ch.
  • Ferrero VE European Commission-Directorate General-Joint Research Centre, Institute for Environment and Sustainability, Ispra, Varese 21027, Italy. valentina.ferrero@jrc.ec.europa.eu.
  • Lettieri T European Commission-Directorate General-Joint Research Centre, Institute for Environment and Sustainability, Ispra, Varese 21027, Italy. Teresa.lettieri@jrc.ec.europa.eu.
  • Colpo P European Commission-Directorate General-Joint Research Centre, Institute for Health and Consumer Protection, Ispra, Varese 21027, Italy. pascal.cplpo@ec.europa.eu.
  • Follonier S CSEM Landquart, Bahnhofstrasse 1, Landquart 7302, Switzerland. stephane.follonier@csem.ch.
  • Calzolai L European Commission-Directorate General-Joint Research Centre, Institute for Health and Consumer Protection, Ispra, Varese 21027, Italy. luigi.calzolai@jrc.ec.europa.eu.
  • Varani L Institute for Research in Biomedicine, Universita' della Svizzera italiana (USI) via Vela 6, 6500 Bellinzona, Switzerland. luca.varani@irb.usi.ch.
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  • 2015-03-04
Published in:
  • International journal of environmental research and public health. - 2015
Biosensing Techniques
Endocrine Disruptors
Environmental Monitoring
Environmental Pollutants
Receptors, Estrogen
English The estrogen receptor protein (ER) can bind a vast number of organic pollutants widely spread in the environment and collectively known as Endocrine Disrupting Chemicals, EDCs. Its broad selectivity makes it an ideal bio-recognition element for the detection of EDCs. Here we describe the strategy and rationale for the design of ER based biosensors and assays that generate a signal in the presence of EDCs. The opportunity to use either natural or rationally modified ER molecules is discussed. The latter approach was successfully applied in the EU-FP7 project RADAR, with the aim to develop a novel biosensor for the detection of organic pollutants both in the environment and in commercial water products.
Language
  • English
Open access status
gold
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https://sonar.ch/global/documents/62396
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