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ABCB1 transporter discriminates human resting naive B cells from cycling transitional and memory B cells.
Journal article

ABCB1 transporter discriminates human resting naive B cells from cycling transitional and memory B cells.

  • Wirths S Institute for Research in Biomedicine, Bellinzona, Switzerland. stefan.wirths@irb.unisi.ch
  • Lanzavecchia A
  • 2005-11-01
Published in:
  • European journal of immunology. - 2005
ATP Binding Cassette Transporter, Subfamily B, Member 1
Adult
B-Lymphocyte Subsets
Cell Cycle
Cell Differentiation
Cells, Cultured
Humans
Immunologic Memory
Infant, Newborn
Resting Phase, Cell Cycle
English The exact identification of B cell subsets is instrumental to understand their dynamics under physiological and pathological conditions. Human memory B cells are currently identified according to the expression of CD27, which is absent on naive B cells. We report here that the ATP-binding cassette (ABC)B1 transporter is exclusively present on mature CD27- naive B cells, while it is absent in CD27+ memory B cells and in a heterogeneous subset of CD27- cells that comprise both switch memory and transitional B cells. Thus, ABCB1 activity precisely discriminates naive from transitional and all memory B cells. Using this improved method to discriminate human B cell subsets, and Ki67 staining to identify recently divided cells, we show that in both cord blood and adult peripheral blood, mature naive B cells are quiescent while transitional B cells and memory B cells have a high in vivo turnover.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/62546
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