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Prevalence of Bacteroides and Prevotella spp. in ulcerative colitis.

  • Lucke K Institute of Medical Microbiology, Canton Hospital Luzern, Switzerland.
  • Miehlke S Medical Department I, University Hospital Dresden, Germany.
  • Jacobs E Institute of Medical Microbiology and Hygiene, Technical University Dresden, Germany.
  • Schuppler M Laboratory of Food Microbiology, Institute of Food Science and Nutrition, Swiss Federal Institute of Technology Zurich, ETH Center LFV B21, Schmelzbergstrasse 7, CH-8092 Zurich, Switzerland.
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  • 2006-04-06
Published in:
  • Journal of medical microbiology. - 2006
Bacteroides
Biopsy
Colitis, Ulcerative
Colonoscopy
DNA, Bacterial
DNA, Ribosomal
Humans
Intestinal Mucosa
Molecular Sequence Data
Prevotella
RNA, Ribosomal, 16S
Sequence Analysis, DNA
English The resident bacterial flora of the large intestine has become increasingly recognized as an essential component in the pathogenesis of ulcerative colitis (UC). However, it is still not known whether the bacterial flora in general or certain bacterial species of the intestinal microbial flora contribute to the pathogenesis of the disease. In order to investigate the composition of the mucosa-associated microbial flora in UC, mucosal tissue samples from patients with active UC and from control subjects with non-inflammatory conditions were analysed and compared. To cover the whole spectrum of intestinal bacteria and to circumvent the known bias introduced by culture-based techniques, comparative 16S rRNA gene sequence analysis was used to determine the bacterial composition in the mucosal tissue samples. The investigation revealed an abundance of sequences from Bacteroides spp. and Prevotella spp. in the mucosal tissue of patients with UC compared with individuals showing no signs of disease. The higher incidence of populations of members of the Bacteroidetes in UC suggests that these may have an influence on the pathogenesis of the disease.
Language
  • English
Open access status
bronze
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Persistent URL
https://sonar.ch/global/documents/64582
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