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DNA typing of HLA-DR beta chain genes can discriminate between undetected alleles and real homozygotes.
Journal article

DNA typing of HLA-DR beta chain genes can discriminate between undetected alleles and real homozygotes.

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  • 1987-01-01
Published in:
  • Immunogenetics. - 1987
Alleles
Cell Line
DNA
DNA Restriction Enzymes
Genes
HLA-D Antigens
HLA-DR Antigens
Haplotypes
Homozygote
Humans
Major Histocompatibility Complex
Nucleic Acid Hybridization
English The polymorphism of HLA-DR antigens has been studied by Southern blot hybridization under conditions specific for the detection of the DR beta chain genes. Haplotype-specific patterns were defined with DNA from DR1, 2, 3, 4, 7, w8, w11, w12, and W13 homozygous typing cells, with restriction enzymes Eco RI, Bgl I, and Pvu II. Certain serological specificities, such as DR2, DR3, and DR7, can be encoded by distinct allelic forms of DR beta chain genes. The procedure of "DNA typing" was applied to family analysis of individuals expressing only a single DR specificity upon serological typing. Three cases are described here: (1) in family GR, phenotypic DR 7 homozygotes correspond to genomic heterozygotes, and a novel DR7 allele is described: (2) in family RU, the genes corresponding to a serologically undetected (blank) DR allele were identified by restriction fragment length polymorphism (RFLP); this novel DR haplotype has an RFLP pattern similar to those of the DRw52 family, even though this specificity was not expressed on the DR-blank lymphocytes; (3) in family RG, there is no blank allele, but a homozygote RFLP situation at the DR subregion.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/64919
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