Use of an isothermal microcalorimetry assay to characterize microbial oxalotrophic activity.
- Bravo D Laboratory of Microbiology, Institute of Biology, University of Neuchâtel, Neuchâtel, Switzerland.
- Braissant O
- Solokhina A
- Clerc M
- Daniels AU
- Verrecchia E
- Junier P
- 2011-06-24
Published in:
- FEMS microbiology ecology. - 2011
Bacteria
Calorimetry
Cupriavidus necator
Oxalates
Soil
Soil Microbiology
Soil Pollutants
Streptomyces
English
Isothermal microcalorimetry (IMC) has been used in the past to monitor metabolic activities in living systems. A few studies have used it on ecological research. In this study, IMC was used to monitor oxalotrophic activity, a widespread bacterial metabolism found in the environment, and particularly in soils. Six model strains were inoculated in solid angle media with K-oxalate as the sole carbon source. Cupriavidus oxalaticus, Cupriavidus necator, and Streptomyces violaceoruber presented the highest activity (91, 40, and 55 μW, respectively) and a maximum growth rate (μmax h(-1) ) of 0.264, 0.185, and 0.199, respectively, among the strains tested. These three strains were selected to test the incidence of different oxalate sources (Ca, Cu, and Fe-oxalate salts) in the metabolic activity. The highest activity was obtained in Ca-oxalate for C. oxalaticus. Similar experiments were carried out with a model soil to test whether this approach can be used to measure oxalotrophic activity in field samples. Although measuring oxalotrophic activity in a soil was challenging, there was a clear effect of the amendment with oxalate on the metabolic activity measured in soil. The correlation between heat flow and growth suggests that IMC analysis is a powerful method to monitor bacterial oxalotrophic activity.
- Language
-
- English
- Open access status
- bronze
- Identifiers
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- DOI 10.1111/j.1574-6941.2011.01158.x
- PMID 21696406
- Persistent URL
- https://sonar.ch/global/documents/659
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