Journal article
Immunization with Naked DNA Coexpressing Antigen and Cytokine via IRES.
- Heinrich J Institute of Medical Virology, University of Zurich, Zurich, Switzerland.
- Strack B
- Nawrath M
- Pavlovic J
- Moelling K
- 2011-03-05
Published in:
- Methods in molecular medicine. - 2000
English
Inoculation of plasmid DNA vectors encoding immunogenic proteins induce humoral as well as cell-mediated immune responses. Protection against challenge with pathogens has provided protective immunity in several instances in animal models. (1,2). DNA vaccines allow the simultaneous expression of antigens and immune-stimulatory cytokines via an internal ribosomal entry site (IRES). Here we describe the construction of a DNA vaccine against malignant melanomas using: (i) the tumor-associated antigen gp100 (or pmel17), known to be over-expressed in many malignant melanomas (3,4), and (ii) the granulocyte macrophage stimulating factor (GM-CSF) which has been shown to have a stimulatory effect on humoral and cellular immune responses (5).
- Language
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- English
- Open access status
- closed
- Identifiers
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- DOI 10.1385/1-59259-688-6:335
- PMID 21374333
- Persistent URL
- https://sonar.ch/global/documents/65959
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