Chromosomal assignment of canine THADA gene to CFA 10q25.
- Soller JT Centre for Human Genetics, University of Bremen, Leobener Straße ZHG, 28359 Bremen, Germany.
- Beuing C Small Animal Clinic and Research Cluster of Excellence "REBIRTH", University of Veterinary Medicine Hanover, Bischofsholer Damm 15, 30173 Hannover, Germany.
- Murua Escobar H Centre for Human Genetics, University of Bremen, Leobener Straße ZHG, 28359 Bremen, Germany.
- Winkler S Centre for Human Genetics, University of Bremen, Leobener Straße ZHG, 28359 Bremen, Germany.
- Reimann-Berg N Centre for Human Genetics, University of Bremen, Leobener Straße ZHG, 28359 Bremen, Germany.
- Drieschner N Centre for Human Genetics, University of Bremen, Leobener Straße ZHG, 28359 Bremen, Germany.
- Dolf G Institute of Genetics, Vetsuisse Faculty, University of Berne, Bremgartenstrasse 109a, PO Box 8466, 3001 Bern, Switzerland.
- Schelling C Department of Animal Science, Swiss Federal Institute of Technology Zurich, Vetsuisse-Faculty Zurich, University of Zurich, Winterthurerstrasse 204, 8057 Zürich, Switzerland.
- Nolte I Small Animal Clinic and Research Cluster of Excellence "REBIRTH", University of Veterinary Medicine Hanover, Bischofsholer Damm 15, 30173 Hannover, Germany.
- Bullerdiek J Centre for Human Genetics, University of Bremen, Leobener Straße ZHG, 28359 Bremen, Germany.
- 2008-06-05
Published in:
- Molecular cytogenetics. - 2008
English
BACKGROUND
Chromosomal translocations affecting the chromosome 2p21 cluster in a 450 kb breakpoint region are frequently observed in human benign thyroid adenomas. THADA (thyroid adenoma associated) was identified as the affected gene within this breakpoint region. In contrast to man tumours of the thyroid gland of dogs (Canis lupus familiaris) constitute mainly as follicular cell carcinomas, with malignant thyroid tumours being more frequent than benign thyroid adenomas. In order to elucidate if the THADA gene is also a target of chromosomal rearrangements in thyroid adenomas of the dog we have physically mapped the canine THADA gene to canine chromosome 10.A PCR was established to screen a canine genome library for a BAC clone containing the gene sequence of canine THADA. Further PCR reactions were done using the identified BAC clone as a template in order to verify the corresponding PCR product by sequencing.Canine whole blood was incubated with colcemid in order to arrest the cultured cells in metaphases. The verified BAC DNA was digoxigenin labeled and used as a probe in fluorescence in situ hybridization (FISH). Ten well spread metaphases were examined indicating a signal on canine chromosome 10 on both chromatids. A detailed fine mapping was performed indicating the canine THADA gene locus on the q-arm of chromosome 10.
RESULTS
The canine THADA gene locus was mapped on chromosome 10q25. Our mapping results obtained in this study following the previously described nomenclature for the canine karyotype.
CONCLUSION
We analysed whether the THADA gene locus is a hotspot of canine chromosomal rearrangements in canine neoplastic lesions of the thyroid and in addition might play a role as a candidate gene for a possible malignant transformation of canine thyroid adenomas. Although the available cytogenetic data of canine thyroid adenomas are still insufficient the chromosomal region to which the canine THADA has been mapped seems to be no hotspot of chromosomal aberrations seen in canine thyroid adenomas.
Chromosomal translocations affecting the chromosome 2p21 cluster in a 450 kb breakpoint region are frequently observed in human benign thyroid adenomas. THADA (thyroid adenoma associated) was identified as the affected gene within this breakpoint region. In contrast to man tumours of the thyroid gland of dogs (Canis lupus familiaris) constitute mainly as follicular cell carcinomas, with malignant thyroid tumours being more frequent than benign thyroid adenomas. In order to elucidate if the THADA gene is also a target of chromosomal rearrangements in thyroid adenomas of the dog we have physically mapped the canine THADA gene to canine chromosome 10.A PCR was established to screen a canine genome library for a BAC clone containing the gene sequence of canine THADA. Further PCR reactions were done using the identified BAC clone as a template in order to verify the corresponding PCR product by sequencing.Canine whole blood was incubated with colcemid in order to arrest the cultured cells in metaphases. The verified BAC DNA was digoxigenin labeled and used as a probe in fluorescence in situ hybridization (FISH). Ten well spread metaphases were examined indicating a signal on canine chromosome 10 on both chromatids. A detailed fine mapping was performed indicating the canine THADA gene locus on the q-arm of chromosome 10.
RESULTS
The canine THADA gene locus was mapped on chromosome 10q25. Our mapping results obtained in this study following the previously described nomenclature for the canine karyotype.
CONCLUSION
We analysed whether the THADA gene locus is a hotspot of canine chromosomal rearrangements in canine neoplastic lesions of the thyroid and in addition might play a role as a candidate gene for a possible malignant transformation of canine thyroid adenomas. Although the available cytogenetic data of canine thyroid adenomas are still insufficient the chromosomal region to which the canine THADA has been mapped seems to be no hotspot of chromosomal aberrations seen in canine thyroid adenomas.
- Language
-
- English
- Open access status
- gold
- Identifiers
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- DOI 10.1186/1755-8166-1-11
- PMID 18522714
- Persistent URL
- https://sonar.ch/global/documents/66877
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