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Matrix-assisted laser desorption/ionization mass spectra reflect solution-phase zinc finger peptide complexation.
Journal article

Matrix-assisted laser desorption/ionization mass spectra reflect solution-phase zinc finger peptide complexation.

  • 1999-01-15
Published in:
  • Journal of the American Society for Mass Spectrometry. - 1999
Amino Acid Sequence
Circular Dichroism
Gene Products, gag
HIV-1
Humans
Molecular Sequence Data
Oxidation-Reduction
Peptides
Protein Precursors
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Zinc Fingers
English The complexation between an 18-residue zinc finger peptide of CCHC type (CCHC = Cys-X2-Cys-X4-His-X4-Cys, X = variable amino acid) from the gag protein p55 of human immunodeficiency virus type 1 (HIV-1) and various transition metal ions was studied by means of circular dichroism spectroscopy and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). A correlation between the complexation behavior in solution and in MALDI-MS could be established. It was shown that MALDI-MS is a fast method suitable for studying metal binding properties of zinc finger complexes.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/66880
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